Tag

Part:BBa_J54025:Design

Designed by: Yusaku Nakashima   Group: iGEM06_Tokyo_Alliance   (2006-10-16)

SuffixI_BglII,MluI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As Suffix of Tokyo Standard.


Tokyo Standard consists of the chain of restriction sites : SalI-BamHI-part-BglII-MluI


BamHI sequence is "G^GATCC", and BglII sequence is "A^GATCT".

They have the sequence "GATC" in common, so you can assemble parts just like Biobrick assembly.

Source

DNA synthesis

References